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High Quality TCEP-HCL CAS:51805-45-9 Tris(2-carboxyethyl)phosphine hydrochloride

High Quality TCEP-HCL CAS:51805-45-9 Tris(2-carboxyethyl)phosphine hydrochloride

Tris(2-carboxyethyl)phosphine hydrochloride CAS:51805-45-9,usually called TCEP-HCL,is a pharmaceutical intermediates, chemical reagent; Reducing agent buffer

  • model no. :

    S0178
  • CAS No : 

    51805-45-9
  • Formula : 

    C9H16ClO6P
  • Weight : 

    286.647
  • Assay : 

    ≥98.0%
  • Appearance : 

    White crystal
  • Brand:

    YACOO
  • port of dispatch :

    Shanghai Port
  • Payment :

    T/T in advance
  • original region :

    China
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  • product description

Description


TCEP HCl is a reducing agent suitable for protein applications. TCEP is often used for the reduction of disulfide bonds within proteins.  This versatile compound is very soluble in aqueous solutions (310 grams/L in water). TCEP is stable in both acidic and basic solutions (pH 1.5 to 8.5).

TCEP HCL has many other advantages such as:
TCEP HCl is stable in air.
TCEP HCl is odorless eliminating the need to preform reactions within a fume hood.
TCEP HCl is efficient and can be used to reduce disulfide bonds at low concentrations (5 mM).
TCEP HCl may be used as a substitute for DTT (dithiothreitol) at a final concentration of 50 mM.


Suzhou Yacoo Science Corporation product TCEP HCl (Item No: S0178) has mature synthetic process with high quality. The purity of TCEP HCl can be made more than 98%. We can provide the products from grams to kilograms,Tris(2-carboxyethyl)phosphine hydrochloride is one of our most advantages diagnostic reagent products.

Specifications
Product Name: Tris(2-carboxyethyl)phosphine hydrochloride 
Short Name:TCEP HCl
CAS No: 51805-45-9
Yacoo Item No: S0178
Formula: C9H16ClO6P
Molecular Weight: 286.647    
Appearance: White crystal
Assay: ≥98.0%
Usage:  TCEP-HCL is a pharmaceutical intermediates, chemical reagent; Reducing agent buffer
Storage:2-8℃
Packing: 25kg/Drum, or as your request



English NameTris(2-carboxyethyl)phosphine hydrochloride

Molecular FormulaC9H16ClO6P

Molecular Wt286.65

CAS51805-45-9

Shelf life: two years

Storage2-8℃

SPECIFICATION PROPERTIES

STANDARD

Appearance

White crystal

Assay,%

≥98.0

MP,℃

170.0-180.0




What is the difference between DTT and TCEP-HCl in the reduction of disulfide bond in protein?


Disulfide bond (−S−S− bond) is a kind of covalent bond between S atoms. It plays an important role in the formation of three-dimensional structure of protein molecules. In order to identify the primary structure of protein, the disulfide bond must be opened firstly. DTT and TCEP-HCl are commonly used for the reduction of disulfide bond, then what is the difference between them?


Difference between DTT and TCEP

 

Prducts

DTT

TCEP

Smell

Slight sulfur smell

No

Soluble

50 g/L

310 g/L

reducibility

3-7 days

2-3 weeks

stability

Week volatility,in the presence of Ni2 it is rapid oxidized

Non-volatile,anti-air oxidation; not affected by metal ions.

pH

6.5-9.0

1.5-8.5

working concentration

1-100mM

5-50mM(most systems)

protein tagged with “His”

avoid

apply

Maleimide labeled  protein reaction

Removed before marking

Non-removed before marking

 



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