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HEPES CAS 7365-45-9

HEPES (4-hydroxyethylpiperazine ethanesulfonic acid) is a non-ionic amphoteric buffer that is highly polar and inert to a wide range of chemicals and enzymes.

Catalog No. :
Q0005
CAS No :
7365-45-9
Empirical Formula :
C8H18N2O4S
Molecular Weight :
238.305
Assay :
≥99.5%
Appearance :
White crystalline powder
Brand:
YACOO
original region :
China

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product description

Synonym(s)：4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula：C8H18N2O4S
CAS Number：7365-45-9
EC Number：230-907-9
Molecular Weight：238.31
Assay：99.5%
Catalog Number：Q0005
Retest period：three years

ANALYSIS	SPECIFICATIONS
Appearance	Crystalline white powder
Assay(%)	99.5% Min.
Loss on Drying	≤0.5%
Solubility In H₂O	(g/L)
pH (1%) @ 20°C	4.7-6.0
pK_a
Heavy Metal	≤5 ppm
Ultraviolet Absorbance (0.1M)	≤0.065
Ultraviolet absorbance (0.05M)	≤0.055
Iron (Fe)	≤5 ppm
Sulfate	≤10 ppm
Endotoxin	≤ 5 EU/g
Residue on Ignition	≤0.1
Infrared Spectrum

Description
HEPES is an organic zwitterionic buffering agent used in biological sciences to maintain pH in solutions. HEPES has buffering capacity in the pH range of 7.2 - 7.4, which is optimal physiological pH for most cells.

Application
HEPES is common buffer used in a wide variety of applications including cell culture, tissue culture, protein purification and extraction, immunoprecipitation, cell lysis, live cell imaging and other biological/biochemical researches. HEPES is used as a Good’s buffer because it has Low absorption spectra in UV, minimal reactivity, stable pH and high water-solubility.

Package Size
25kg Drum/Pail
Customized packaging sizes are available

Storage/Shipping
Ship and store in Ambient Temperature

Shelf Life/Retest
2 years from the date of manufacture

For Research and Development Use Only. Not approved for use in clinical or in vitro diagnostic procedures.

Related Tags :

HEPES 7365-45-9 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid(HEPES)

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