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HEPES  CAS 7365-45-9

HEPES CAS 7365-45-9

HEPES (4-hydroxyethylpiperazine ethanesulfonic acid) is a non-ionic amphoteric buffer that is highly polar and inert to a wide range of chemicals and enzymes.

  • Catalog No. :

    Q0005
  • CAS No : 

    7365-45-9
  • Empirical Formula : 

    C8H18N2O4S
  • Molecular Weight : 

    238.305
  • Assay : 

    ≥99.5%
  • Appearance : 

    White crystalline powder
  • Brand:

    YACOO
  • original region :

    China
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  • product description
Synonym(s):4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
Empirical Formula:C8H18N2O4S
CAS Number:7365-45-9
EC Number:230-907-9
Molecular Weight:238.31
Assay:99.5%
Catalog Number:Q0005
Retest period:Two years

ANALYSIS

SPECIFICATIONS

Appearance

Crystalline white powder

Assay(%)

99.5% Min.

Loss on Drying

0.5%

Solubility In H2O

(g/L)

pH (1%) @ 20°C

4.7-6.0

pKa

 

Heavy Metal

5 ppm

Ultraviolet Absorbance (0.1M)

0.065

Ultraviolet absorbance (0.05M)

0.055

Iron (Fe)

5 ppm

Sulfate

10 ppm

Endotoxin

5 EU/g

Residue on Ignition

0.1

Infrared Spectrum



Description    
HEPES is an organic zwitterionic buffering agent used in biological sciences to maintain pH in solutions. HEPES has buffering capacity in the pH range of 7.2 - 7.4, which is optimal physiological pH for most cells.

Application    
HEPES is common buffer used in a wide variety of applications including cell culture, tissue culture, protein purification and extraction, immunoprecipitation, cell lysis, live cell imaging and other biological/biochemical researches. HEPES is used as a Good’s buffer because it has Low absorption spectra in UV, minimal reactivity, stable pH and high water-solubility.

Package Size    
25kg Drum/Pail
Customized packaging sizes are available

Storage/Shipping    
Ship and store in Ambient Temperature

For Research and Development Use Only.  Not approved for use in clinical or in vitro diagnostic procedures.

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