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High purity PIPES disodium salt CAS: 76836-02-7   Disodium piperazine-1,4-diethanesulphonate High purity PIPES disodium salt CAS: 76836-02-7   Disodium piperazine-1,4-diethanesulphonate

High purity PIPES disodium salt CAS: 76836-02-7 Disodium piperazine-1,4-diethanesulphonate

PIPES [piperazine-N,N′-bis(2-ethanesulfonic acid)] is frequently used as a buffering agent in biochemistry; it is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has a pKa near the physiological pH which makes it useful in cell culture work. It has been documented that PIPES disodium salt may help prevent lipid loss when buffering glutaraldehyde-fixed histological samples.
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    White crystalline powder
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  • product description

Suzhou Yacoo Science Corporation was founded in 2003, specialized in Biological buffers, Diagnostic reagents, Medical intermediates and Electronic chemicals. We have our factory in Shandong Province to offer high-quality and low-price PIPES disodium salt for Yacoo customers.


English NamePiperazine-1,4-bis(2-ethanesulfonic acid) disodium salt

Molecular FormulaC8H16N2O6S2Na2

Molecular Wt346.33


Shelf lifetwo years




White crystalline powder



Solubility20% aq.

Clear colorless solution

Loss on drying,%


PH (1% aq.)


Ultraviolet absorbance0.1M aq.


Ultraviolet absorbance0.1M aq.



PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized.

 Since its pKa at 37°C is near physiological pH, it has applications in cell culture work. Sigma offers a Biotechnology Performance Certified Product (P1851), as well as several different salts for convenience in buffer preparation.

Buffers can be prepared by adding a solution of base to PIPES free acid, titrating to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt, titrating to the appropriate pH.

Storageroom temperature

Packing: 25kg/Drum, or as your request

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