3,3',5,5'-Tetramethylbenzidine(TMB) is a highly sensitive and important in vitro diagnostic reagent, mainly used in enzyme-linked immunosorbent assay (ELISA), immunospot hybridization or immunohistochemistry, chlorine and peroxide detection and analysis of hydrogen.

Jinyang Chen, etc. have established a rapid detection method for glutathione of the present invention based on TMB, the detection method is specifically: chitosan solution, H2O2 solution and 3,3',5,5'-Tetramethylbenzidine solution is mixed and reacted, and then glutathione solutions with different concentration gradients are added to the mixed reaction system to continue the reaction. After the reaction is completed, the absorbance of the solution is measured by an ultraviolet spectrophotometer , Construct a linear relationship between absorbance and glutathione concentration, and use this linear relationship to quickly detect the concentration of glutathione in the solution to be tested; or you can add glutathione with different concentration gradients through visual observation and camera photography After the color of the solution, use the Image J software to measure the photographed pictures to obtain the red, green and blue color averages of different solution pictures, so as to realize semi-quantitative analysis of the concentration of glutathione in the test solution.

Compared with other chromogens, TMB has higher sensitivity and is non-carcinogenic. It is used to detect glutathione and has important application value for biomedical research and clinical medical testing.

References: Chen Jinyang, Sun Mengtong, Wang Yuxin, Ku Lingyue, Liu Xiangru, A rapid detection method for glutathione, CN110907446A