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99.5% purity HEPES CAS:7365-45-9 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid 99.5% purity HEPES CAS:7365-45-9 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid

99.5% purity HEPES CAS:7365-45-9 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid

HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) is a zwitterionic organic chemical buffering agent; one of the twenty Good's buffers.

  • model no. :

    Q0005
  • Brand:

    YACOO
  • port of dispatch :

    Shanghai Port
  • Payment :

    T/T in advance
  • original region :

    China
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  • product description

Suzhou Yacoo Science Corporation was founded in 2003, specialized in Biobuffer, Diagnostic reagents, Medical intermediates and Electronic chemicals. We have our factory in Shandong Province to offer high-quality and low-price HEPES for Yacoo customers.


Specification:

English Name4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid

Molecular FormulaC8H18N2O4S

Molecular Wt238.31                                                  

CAS7365-45-9 

Shelf life: two years

SPECIFICATION PROPERTIES

STANDARD

Appearance

White crystalline powder

Assay,% 

99.0101.0

Solubility (1M aq.)

            Clear, colorless solution

PH (1% aq.)

4.7-6.0

Heavy Metals,ppm

≤5

Loss on drying,% 

≤0.5

Ultraviolet absorbance1 M aq.

≤0.065

Ultraviolet absorbance1 M aq.

≤0.055

 Iron ion, ppm

≤5

Sulfate Ion,ppm

≤10

Ignition residue,%

≤0.1


Usage: HEPES is widely used in cell culture, largely because it is better at maintaining physiological pH despite changes in carbon dioxide concentration (produced by cellular respiration) when compared to bicarbonate buffers, which are also commonly used in cell culture. The dissociation of water decreases with falling temperature, but the dissociation constants (pK) of many other buffers do not change much with temperature. HEPES is like water in that its dissociation decreases as the temperature decreases. This makes HEPES a more effective buffering agent for maintaining enzyme structure and function at low temperatures. Lepe-Zuniga et al. reported a phototoxicity of HEPES when exposed to ambient light by the production of hydrogen peroxide, which is not a problem in bicarbonate-based cell culture buffers. It is therefore strongly advised to keep HEPES-containing solutions in darkness as much as possible.

Storageroom temperature

Packing: 25kg/Drum, or as your request


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