CHAPS is an organic compound with molecular formula C₃₂H₅₈N₂O₇S and molecular weight 614.877. CHAPS is a kind of zwitterionic detergent, widely used in protein purification process. CHAPS can also be used in biochemical research to denature and split cells, extract RNA and DNA; In addition, CHAPS can also be used for the preparation of buffer solution. CN102796726B patent discloses a method for extracting nucleic acid from mixed microorganisms. This week, YACOO editor briefly introduced the composition of the reagent for cracking mixed microorganisms and the method for extracting nucleic acid from cracking mixed microorganisms.

The reagent for cracking mixed microorganisms consists of extract A and extract B.

Preparation method of extract A

Tris, EDTA, SDS, Tween 80, Triton X-100, Brij 58, CHAPS, sodium dodecyl sarcosine β- Sulfhydryl ethanol and water were mixed to obtain extract A, in which the concentration of CHAPS in extract A was 0.05% - 0.2% (volume percentage);

Preparation method of extract B

The water saturated phenol, ethanol and pyrrolidone were mixed to obtain extraction solution B. The volume ratio of water saturated phenol, ethanol and pyrrolidone was 98:1:1.

Extract A, Carrier RNA and mixed microorganism are mixed, and then mixed with extract B for reaction to obtain reaction solution; Centrifuge and collect the upper water phase to obtain the mixed microbial lysate.

This patent provides an extraction method of mixed microbial nucleic acid, which can simultaneously extract nucleic acid from mixed samples of virus, gram-negative bacteria, gram-positive bacteria, mycoplasma and chlamydia. Its main characteristics are simple operation, low cost and good extraction effect.

Reference

CN102796726B Extraction of nucleic acid from mixed microorganisms.