Tricine (CAS:5704-04-1) is an excellent amphoteric biological good's buffer. The main purpose of Tricine is to effectively inhibit Gram-positive bacteria, Gram- negative bacteria, yeasts and molds, and it is also a pH stabilizer for many vaccine culture media. CN110117589B introduced a kind of Tricine in the field of molecular biology technology. The application of saliva preservation solution in this patent is as follows：

EDTA 3-8mmol/L, ethylene glycol-bis-(2-aminoethyl ether)tetraacetic acid 3-8mmol/L, benzyl penicillin sodium salt 40-60μg/mL, streptomycin sulfate 40-60μg/mL, Tris- HCl 40-60mmol/L, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid 5-15mmol/L, Tricine 40-60mmol/L, Proteinase K 5-15μg/mL, Sucrose 5-15mmol/ L, the solvent is sterile water.

4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid and Tricine in the above specific concentration range can synergistically inhibit the activity of nucleases and maintain cell osmotic pressure, which can still maintain DNA after long-term storage. Its integrity and high quality, and can be used for long fragment amplification have major contributions. Tricine also has a broad-spectrum antibacterial effect; proteinase K can degrade nucleases and maintain DNA integrity to the greatest extent; Tris-HCl can maintain the stability of the pH value of the preservation solution; in addition to maintaining the osmotic pressure of cells, sucrose can also increase the viscosity of the preservation solution and protect DNA from breaking.

The components in the saliva preservation solution of the present invention are reasonably matched within a specific concentration range, so that the DNA samples stored in the saliva preservation solution for a long time at room temperature can maintain their integrity, and can be directly used for PCR amplification without DNA extraction; it can be used for NGS experiments, It can adapt to complex multiplex PCR experiments and meet the needs of long fragment amplification.

References

CN110117589B A saliva preservation solution and its preparation method and application.