Morpholinic buffers——MES,MOPS and MOPSO

Morpholinic buffers——MES, MOPS and MOPSO

2017-11-28

The zwitterionic buffer, also known as Good's Buffer, is a series of N-substituted sulfamic acids that have good pH stability and are inert to a variety of chemicals and enzymes. Among them, the morpholinic buffers, including MES, MOPS and MOPSO, they do not form complexes with most metal ions and are therefore suitable for solutions containing metal ions. But what are the specific experiments they used in? This article will summarize for everyone.

MES

Its chemical name is 2-(N-morpholino) ethanesulfonic acid, molecular formula C6H13NO4S, pKa 6.1 at 25°C, pH buffer range 5.5~6.7. The specific available experiments are as follows:

(1) Biological buffer, can be used to replace highly toxic cacodylate, and ion buffer citrate and malate;
(2) Buffered media commonly used for bacterial, yeast and mammalian cells. High concentrations of MES are toxic to most plants, but can be used in plant media at concentrations ~10 mM;
(3) as a buffer for studying Tau protein in culture;
(4) Because of its low ion mobility and low conductivity at high concentrations, it can be used in many types of electrophoresis and chromatography including capillary electrochromatography, gel filtration chromatography, phosphocellulose column chromatography, hydrophobic interaction chromatography, cation exchange chromatography and SDS-PAGE;

(5) Complex with organotin (IV) molecules as antitumor agents.

MOPS

Its chemical name is 3-(N-morphine) Propanesulfonic acid, molecular formula C7H15NO4S, pKa 7.14 at 25°C, pH buffer range is 6.5~7.9. Specific available experiments are as follows:

(1) as running buffer in electrophoresis and for protein purification in chromatography;
(2) Formulated into a variety of agar medium for the culture of bacteria, yeast and mammalian cells. However, concentrations above 20 mM are not recommended for use in mammalian cells;
(3) Lysis buffer that can be used for Escherichia coli cells;
(4) as eluent in gel filtration chromatography;
(5) Applied to Northern blot as a buffer for RNA isolation and transfection;
(6) For bicinchoninic acid (BCA) assay;

(7) Studies on electron transport and phosphorylation of chloroplast sample preparation.

MOPSO

Its chemical name is 3-(N-morpholino)-2-hydroxypropanesulfonic acid, molecular formula C7H15NO5S, pKa 6.87 at 25°C, the pH buffer range 6.2~7.6. Specific available experiments are as follows:

(1) use as a carrier electrolyte in capillary electrophoresis, and as a crystallization buffer for glutathione synthetase;
(2) used in fluorescence spectrometry, spectrophotometry and isothermal titration calorimetry;
(3) Interaction with the peptide backbone of bovine serum albumin (BSA) to prevent thermal denaturation of BSA;
(4) as one of the buffer components for copper correlation analysis;
(5) used as a buffer carbon yeast extract medium composition;
(6) MOPSO-ethanol buffer system was prepared to fix urine-derived cells;

(7) A buffer system for the determination of biological by-products of marine crude oil.

Although Good's buffer has the advantages of not participating in and not interfering with the biochemical reaction process, not inhibiting the enzymatic chemical reaction, and not reacting with the metal ion. However, in the specific use, there are still some differences in the application range of several morpholine series buffers MES, MOPS and MOPSO. Therefore, before carrying out the experiment, it is necessary to consider the buffer requirements and specific experimental types so as to select the most appropriate buffer.

Edited by Suzhou Yacoo Science Co., Ltd.

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