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Guanidine hydrochloride CAS 50-01-1
Guanidinium chloride or guanidine hydrochloride, usually abbreviated GuHCl and sometimes GdnHCl or GdmCl, is the hydrochloride salt of guanidine.
TRIS (Tromethamine) Pharmaceutical Grade CAS 77-86-1
Tris is widely used in the preparation of buffers in biochemistry and molecular biology experiments. In addition, Tris is the intermediate for the preparation of surfactants, vulcanization accelerators and some drugs.
HEPES CAS 7365-45-9
HEPES (4-hydroxyethylpiperazine ethanesulfonic acid) is a non-ionic amphoteric buffer that is highly polar and inert to a wide range of chemicals and enzymes.
MOPS CAS 1132-61-2
3 - morpholine propyl sulfonic acid is a kind of biological buffer, used in biochemical diagnostic kit, DNA/RNA extraction kit and PCR diagnostic kits
N-Cyclohexyl-3-aminopropanesulfonic acid CAS1135-40-6
Biological buffer, used in biochemical diagnostic kit, DNA/RNA extraction kit and PCR diagnostic kits

3,3',5,5'-Tetramethylbenzidine CAS 54827-17-7 TMB Reagent

3,3’,5,5’-Tetramethylbenzidine (TMB) is a chemical compound with a molecular formula C₁₆H₂₀N₂. This organic compound is widely utilized in biochemistry and molecular biology as a substrate for the detection of peroxidase activity. TMB undergoes colorimetric changes in the presence of peroxidase, resulting in the development of a blue color. This makes it a crucial component in various enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry applications.

Catalog No. :
S0025
CAS No :
54827-17-7
Empirical Formula :
C16H20N2
Molecular Weight :
240.3434
Assay :
≥99.0%
Appearance :
White or light yellow crystal
Brand:
YACOO
port of dispatch :
shanghai
original region :
Chinese

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product description

Synonym(s)：3,3’,5,5’-Tetramethylbenzidine
Empirical Formula：C16H20N2
CAS Number：54827-17-7
EC Number：259-364-6
Molecular Weight：240.35
Assay：≥99.0%
Catalog Number：S0025

Shelf Life/Retest：2 years

ANALYSIS	SPECIFICATIONS
Appearance	White/ light yellow crystal
Purity (%)	≥99.0
MP ,°C	168.0-171.0
Thin layer chromatography （TLC）	Single spot
Heavy Metal, ppm	≤5
Loss on drying, %	≤0.5

Description
3,3’,5,5’-Tetramethylbenzidine (TMB) is a chemical compound with a molecular formula C16H20N2. This organic compound is widely utilized in biochemistry and molecular biology as a substrate for the detection of peroxidase activity. TMB undergoes colorimetric changes in the presence of peroxidase, resulting in the development of a blue color. This makes it a crucial component in various enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry applications.

Application
TMB's distinctive color reaction is employed to visualize and quantify the presence of specific biological molecules, such as antigens or antibodies, in various assays. The color change occurs due to the oxidation of TMB by peroxidase, leading to the formation of a blue product. The intensity of the color is directly proportional to the amount of peroxidase activity, providing a sensitive and quantitative measure for detecting biological interactions.

Beyond its applications in the field of life sciences, 3,3’,5,5’-Tetramethylbenzidine finds use in other areas, such as analytical chemistry, where it serves as a reagent for the detection of various substances. Its versatility, stability, and reliable colorimetric response make TMB a valuable tool in research and diagnostic settings, contributing to advancements in our understanding of biological processes and disease detection.

A TMB stock solution can be prepared by dissolving 1 mg/ml in DMSO. The working solution is made by diluting 1 ml of the DMSO stock with 9 ml phosphate citrate buffer, pH 5.0, and then adding 2 ml of fresh 30%
hydrogen peroxide per 10 ml of substrate/buffer solution immediately prior to use. Stock solutions of TMB in DMSO can be frozen in 1–2 ml aliquots at –20 °C. Solutions appear to remain active for at least two years when stored in this manner. Any oxidation would be revealed as a color change in
the working solution.

Package Size
500g/bottle, 1kg/bottle, 25kg/drum

Storage/Shipping
2-8ºC in dark

Shelf Life/Retest

2 years

Related Tags :

High Quality 3,3',5,5'-Tetramethylbenzidine CAS 54827-17-7 TMB Diagnostic reagent 3,3',5,5'-Tetramethylbenzidine TMB C16H20N2 3,3',5,5'-Tetramethylbenzidine Reagent

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