3,3',5,5'-Tetramethylbenzidine CAS 54827-17-7 TMB Reagent
3,3’,5,5’-Tetramethylbenzidine (TMB) is a chemical compound with a molecular formula C16H20N2. This organic compound is widely utilized in biochemistry and molecular biology as a substrate for the detection of peroxidase activity. TMB undergoes colorimetric changes in the presence of peroxidase, resulting in the development of a blue color. This makes it a crucial component in various enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry applications.