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Advantages of Tris buffer and precautions for use 2020-09-11 16:49:05

Biological buffers are of great significance in biochemical research, and are widely used in experiments such as western blotting, biochips and biological hybridization, and in vitro diagnostic industries. The biological buffer system contains 20-30 varieties. Yacoo Science's main biological buffer covers the most important products, Such as Tris, HEPES, MOPS, BIS-TRIS, etc. This article will introduce the advantages and precautions of Tris buffer, which is Yacoo Science's superior product.


Tris, full name is tris(hydroxymethyl)aminomethane, its CAS number is77-86-1, pKa at 25°C is 8.06, buffer range 7.0-9.0. Common Tris buffers include Tris-HCl , Tris-phosphate  and a variety of derivative buffers, such as TBS, TE, etc. Tris  is a very important biological buffer, its advantages are as follows:

1. Tris base is relatively alkaline, so this buffer system can be used to prepare a wide range of pH buffers ranging from acidic to alkaline;

2. It has little interference to the biochemical process, and does not precipitate with calcium, magnesium ions and heavy metal ions;

3. It has high solubility in water, and inert to many enzyme reactions;

4. It has high buffering capacity and is generally used to stabilize the pH of the reaction systemand has a strong buffering capacity between pH 7.5-9.0;

5. Tris buffer is widely used and plays an important role in biochemistry, molecular biology, in vitro diagnostics, cosmetics, coatings and other fields.

Things need pay attention to when using Tris buffer:

1. The pH value of Tris buffer is greatly affected by temperature and concentration, so the use environment should be considered during the preparation process;

2. Tris reacts with various molecules to a certain extent, including RNase inhibitors, aldehydes, enzymes, DNA, and common metals such as Cr3+, Fe3+, Ni2+, Co2+ and Cu2+, etc. It can work with heavy metals, but in some systems Also inhibits

3. It is easy to absorb CO2 in the air, and the prepared buffer should be tightly sealed;

4. It has a certain interference effect on some pH electrodes, so it is necessary to use an electrode compatible with Tris solution;

5. Tris is not suitable for bicinchoninic acid (BCA) determination;

6. Inhalation, ingestion, skin absorption can cause injury, wear gloves and goggles during operation.


Yacoo Science’s main products, biological buffer, is a type of alcohol and ammonia fine chemicals with special characteristics, it does not participate in and does not interfere with the biochemical process. It can be used as a buffer system for life science research and provides a stable pH environment for experiments. The products are widely used in in vitro diagnostics, bio-pharmaceuticals, bio-cosmetics, coatings and paints and other industries. and Yacoo's customers include many of the world's top 500 companies.

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