The Good's Buffer is a series of N-substituted sulfamic acids that have good pH stability and are inert to a variety of chemicals and enzymes. 3-(N-morpholino)propanesulfonic acid, referred to as MOPS, is one of Good's Buffer, it plays a very important role in the biological experiments.

What are specific experiments MOPS used in?

The buffer range of MOPS is between 6.5 and 7.9, which is

(1) Suitable for the study of electron transfer and phosphorylation of the chloroplast thin layer;
(2) Can be prepared into a variety of agar medium and used as a non-toxic buffer in Streptomyces culture and cephalosporin production, and Lysis buffer that can be used for Escherichia coli cells;
(3) Can be used as electrolyte system components for isoelectric focusing electrophoresis (IEF) of two-dimensional gel electrophoresis;
(4) Can be used in Northern hybrid, as RNA separation and membrane buffer;

(5) Can be used for bicinchoninic acid (BCA) assay.

Comparison of the buffer range between MOPS (CAS 1132-61-2) and other buffers:

Table 1. The buffering range of MOPS and other buffers.

The preparation method of commonly used 10× MOPS buffer is as follows:

7. Store at room temperature.

MOPS buffer is a zwitterion buffer, if the usage amount is small, it should be properly split charging. For safety and health, experimenter should wear clothing and disposable glove. If accidentally contact with the eyes, the operator should immediately rinse with plenty of water and seek medical attention.