At present, there are more than 120 million people with diabetes in the world. Diabetes has become the third most serious chronic disease that threatens human health after cancer and cardiovascular disease. For patients with diagnosed diabetes, in order to control and delay the development of the disease, reduce the occurrence of complications, blood glucose must be measured regularly and irregularly. How to quickly and accurately detect the glucose content in the blood has positive significance. The researchers developed a glucose oxidase single liquid assay reagent containing MOPS with excellent stability and sensitivity and a wide range of detection wavelengths.
At present, the blood glucose level enzymatic method mainly includes: hexokinase method, glucose dehydrogenase method, oxidase method, enzyme electrode, blood glucose meter, etc. The following problems exist:
Component |
Concentration |
Glucose oxidase |
1-50KU/L |
Peroxidase |
0.5-50KU/L |
4-aminoantipyrine |
0.1-20mmol/L |
Sodium sulfate |
1-200g/L |
MOPS |
5-500mmol/L |
High-sensitivity composite chromogen |
0.05-10g/L |
Preservative |
0.05-20g/L |
Buffer |
pH 5.0-8.0 |
The glucose content can be measured by measuring the absorbance at a wavelength of 500~600 nm under the biochemical analyzer. The invention can be stable for at least one year in the form of a stable single reagent, and the operation is simple and can be conveniently applied, the reagent waste and environmental pollution are reduced. It has good sensitivity and wide detection wavelength range (500-600nm), which can be applied to various biochemical detection instruments and improve instrument utilization.
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