The zwitterionic buffer, also known as Good's Buffer, is a series of N-substituted sulfamic acids that have good pH stability and are inert to a variety of chemicals and enzymes. Among them, the morpholinic buffers, including MOPS Sodium Salt, MES, and MOPSO, have some common character, for example, they do not form complexes with most metal ions and are therefore suitable for solutions containing metal ions. But what is the difference between them?
Let’see the following table:
Table 1. The comparation of MOPS, MES, MOPSO.
Buffer |
Structure |
Full Name |
Formula |
pKa(25°C) |
Buffering range |
MOPS |
|
3-(N-morphine) Propanesulfonic acid |
C7H15NO4S |
7.14 |
6.5~7.9 |
MES |
|
2-(N-morpholino) ethanesulfonic acid |
C6H13NO4S |
6.1 |
5.5~6.7 |
MOPSO |
|
3-(N-morpholino)-2-hydroxypropanesulfonic acid |
C7H15NO5S |
6.87 |
6.2~7.6 |
The application of MOPS, MES and MOPSO:
MOPS:
Although Good's buffer has the advantages of not participating in and not interfering with the biochemical reaction process, not inhibiting the enzymatic chemical reaction, and not reacting with the metal ion. However, in the specific use, there are still some differences in the application range of several morpholine series buffers MES, MOPS (CAS 1132-61-2) and MOPSO. Therefore, it is necessary to consider the buffer requirements and specific experimental types so as to select the most appropriate buffer.
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