As we all know, guanidine hydrochloride is often used as an intermediate in medicines, pesticides, dyes and other organic compounds, it is also an important raw material for sulfonamides and folic acid; it can be used as a strong denaturants in the extraction of total RNA from cells. At the same time, guanidine hydrochloride is often used in various buffers for biological experiments. Let us take a look at the following recipes.

• 6 M guanidine hydrochloride
• 8 mM imidazole
• 50 mM NaH2PO4
• 50 mM Tris

Adjust pH to 8.0. Prepare within a few days of the experiment and store at room temperature (20~25°C).

• 6 M Guanidine hydrochloride
• 5 mM DTT
• 50 mM Tris-Cl (pH 7.5)

Warm the solution to 40ºC to dissolve the guanidine HCl. Adjust the pH to 7.5 if necessary.

• Hydroxylamine hydrochloride (Analar grade)
• Guanidine hydrochloride (6 M aqueous), prechilled
• Potassium carbonate (1 M)
• NaOH (50% w/v)

The inclusion of guanidine hydrochloride is reported to improve the efficiency of Asn-Gly cleavage.

• 8 M guanidine hydrochloride
• 50 mM Tris-Cl (pH 7.5)

• 10 mM DTT (dithiothreitol)
• 8 M guanidine hydrochloride
• 50 mM Tris-Cl (pH 8.5)

• Guanidine hydrochloride (6 M)
• Na2HPO4 (0.1 M, pH 8.0)

• 50 mM Sodium phosphate, containing 150 mM NaCl (pH 7.0)
• Denaturing buffer containing urea
• Denaturing buffer containing guanidine hydrochloride
• Peptide elution buffers