Product Introduction IPTG is a highly potent inducer that is widely used in laboratories due to its stability during the induction process, as it is not metabolized by bacteria. IPTG is commonly used as an inducer in biopharmaceuticals and enzyme catalysis technology. Application of IPTG Carbohydrates, also known as carbohydrates, are the main source of energy for our body. Carbohydrates combine with other substances in the body to form enzymes, antibodies, hormones, etc., which are of great significance in regulating the physiological functions of the human body. Most monosaccharides and disaccharides are widely used in dietary products for their good sweet taste and affordable price. Once we directly ingest glucose and other monosaccharides from food, and starch and other high calorie substances that can be decomposed into glucose and other substances in the body are excessive, it will lead to obesity, diabetes, hypertension and a series of adult diseases, endangering health. Rare sugar is a new sweetener for people with diabetes and obesity. It is similar to sucrose, but it has the advantages of low calorie, high stability, harmony between sweetness and dullness, no hygroscopicity, no cariogenicity, and high tolerance. The conversion of high calorie sugars such as glucose in the human body into low calorie, rare sugars that are not directly absorbed by the human body has certain practical significance. Therefore, the CN117987403A patent has developed a method and application based on the catalytic conversion function of biological enzymes to reduce the absorption of high calorie sugars in the body. The screening of its glucose isomerase includes the preparation of recombinant enzymes. The specific method for preparing recombinant enzymes is as follows: Recombinant plasmids pET30a TfGI, pET30a AsGI, and pET30a TtGI were transformed into the host bacteria of Escherichia coli BL21 (DE3), and cultured overnight at 37℃ on LB plates containing Kana. Select the monoclonal transformants and transfer them to 200mL LB liquid medium. Incubate them at 37℃ and 200rpm until OD600=0.6. Add isopropyl βD thiopyranogalactoside (IPTG) inducer with a final concentration of 0.5mM, and induce the culture at 16℃ for 12-16 hours. Centrifuge at 4℃ and 10000rpm for 10 minutes using a high-speed freezing centrifuge to collect bacterial cells. Remove the supernatant from the culture medium and add it to the buffer. Crush the cells with ultrasound and collect the supernatant by centrifugation. This patented technology utilizes various biological enzymes to catalyze the conversion of high calorie sugars into low calorie, non directly absorbed rare sugars by the human body. Through the encapsulation of the drug delivery system, it effectively reduces the damage of the intestinal environment to key enzymes, while utilizing the catalytic effect of key enzymes to reduce the absorption of high calorie sugars by the human body, converting high calorie sugars into low calorie rare sugars; It is beneficial to prevent and treat diabetes and obesity caused by high blood sugar.
References CN117987403A Method and application of reducing high calorie sugar absorption in the body based on the catalytic conversion function of biological enzymes.
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