Product Name：MES

CAS：4432-31-9

Molecular Formula：C₆H₁₃NO₄S

Article No.：M0006

Structural Formula：

Product Introduction

MES is a white solid powder at room temperature and pressure, and its molecular structure contains a morpholine ring and an ethanesulfonic acid group. Morpholine ring is a nitrogen-containing heterocyclic compound with high stability and inertness, and has no obvious toxicity or irritation to living organisms. The ethanesulfonic acid group is a negatively charged functional group with good water solubility and buffering properties. 2-Morpholine ethanesulfonic acid can be used as a component of a buffer and accelerator in cosmetics, biopharmaceuticals, IVD, sealing materials, plant culture and other fields.

Application of MES

Sea buckthorn is an important ecological woody grain and oil tree species for soil and water conservation and sand control, with fast growth, drought resistance, salt alkali resistance, and wind and sand resistance. The fruits, leaves, and stems of seabuckthorn are rich in various bioactive ingredients that are beneficial to the human body, have high nutritional and medicinal value, and are widely used in industries such as food, daily chemical, and medicine. At present, there are few reports on the genetic transformation of seabuckthorn, which has long constrained the development of seabuckthorn genetic engineering research. Therefore, exploring transgenic methods for seabuckthorn and improving the efficiency of functional gene transformation is one of the main problems that urgently need to be solved in seabuckthorn genetic engineering and genetic breeding research. In this context, the CN117867015A patent provides a method for Agrobacterium mediated genetic transformation of seabuckthorn by soaking flowers. The steps for preparing Agrobacterium immersion solution are as follows:

① Add Agrobacterium tumefaciens containing pCAMBIA-1300-mCherry to 5mL YEB liquid medium for activation, and shake at 180 rpm in a shaking table at 28 ℃ for 18-20 hours.

② Add 4mL of activated bacterial solution to 200mL YEB liquid medium (containing 100mg/L rifampicin, 100mg/L Str, 50mg/L kanamycin, 10mmol/L 2-morpholine ethanesulfonic acid, and 150mmol/L acetylsyringane), and incubate at 180rpm on a shaking table at 28 ℃ for approximately 18 hours.

③ Place the bacterial solution obtained in the previous step in a 4℃ centrifuge, centrifuge at a speed of 4000rpm for 15 minutes, discard the supernatant and collect the bacterial body. Resuspend the bacterial body with buffer solution until OD600=0.8-1.0.

Using Agrobacterium LBA4404 containing the mCherry reporter gene to immerse the female inflorescence of seabuckthorn after pollination, transgenic fruits can be obtained. This not only greatly shortens the transformation cycle, but also results in intuitive fluorescence observation and long-term stable expression, providing a fast and reliable method for functional verification of seabuckthorn genes, and laying a foundation for cultivating new transgenic seabuckthorn varieties.

References

CN117867015A  A genetic transformation method for sea buckthorn of agrobacterium mediated flower soaking