Product Name：PIPES

CAS：5625-37-6

Molecular Formula： C₈H₁₈N₂O₆S₂

Article No.：P0002

Structural Formula：

Product Introduction

PIPES is mainly used as a biological buffer and can be used in laboratory research and chemical production processes. 

Application of PIPES

Antithrombin III is mainly synthesized in liver cells and endothelial cells. It is a single chain glycoprotein composed of 432 amino acid residues, with a relative molecular weight of 58200KD. Antithrombin III is thrombin and coagulation factor XIIα、XIα、IXα、Xα Inhibitors of proteases. It combines with thrombin through arginine serine peptide bonds to form an antithrombin thrombin complex, which inactivates thrombin. Heparin can accelerate this reaction by more than a thousand times. Antithrombin III, as the most important inhibitor of active clotting factors in the blood, controls blood coagulation and fibrinolysis. The level of antithrombin III in the blood varies according to various diseases and symptoms. In diseases such as acute myocardial infarction, disseminated intravascular coagulation, liver disease, and nephrotic syndrome, the concentration of antithrombin III decreases. Therefore, the concentration of antithrombin III is of great significance as an indicator for the diagnosis, efficacy monitoring, and prognosis judgment of such diseases. At present, most of the commercially available antithrombin III assay kits are based on the S-2238 substrate method, mostly in freeze-dried dosage forms. The reagent composition is diverse, the production process and operation are complex, and the cost investment is high. Therefore, the CN114544977A patent provides an antithrombin III assay kit. The detection kit includes reagent 1 and reagent 2.

Reagent 1 includes buffer, surfactant, reaction regulator, preservative, and anti-interference agent. The buffer solution in reagent 1 is a 10-200mM buffer with a pH between 6.0-8.0, which can be Tris HCl buffer, Na₂HPO₄ -NaH₂PO₄ buffer, MES buffer, MOPSO- MOPSO/Na buffer, PIPES buffer, HEPES buffer, Na₂HPO₄ -HCl buffer, or Na₂HPO₄ -KH₂PO₄ buffer;

Reagent 2 includes buffer, surfactant, reaction regulator, protein protectant, preservative, and antithrombin III antibody.

This patent improves the uniformity of the reagent by adding surfactants; The addition of anti-interference agents enhances the reaction specificity of the reagent in determining the target substance, thereby improving the accuracy of the reagent determination results; The sensitivity of the reagent was enhanced by adding reaction regulators, which improved the accuracy of low value sample determination results.

References

CN114544977A  A antithrombin III assay kit.