Product Name：MOPS-Na

CAS：71119-22-7

Molecular Formula： C₇H₁₄NNaO₄S

Article No.：M0002

Structural Formula：

Product Introduction

MOPS-Na is commonly used as a buffer in biology. In biological experiments, it is an important pH stabilizing reagent, and appropriate weak acids and their conjugated bases are usually selected to mix to obtain a suitable pH value. Most biological reactions occur under neutral conditions, with pH generally selected between 6 and 8.

Application of MOPS-Na

Ebomycin is a highly promising class of anti-tumor drugs. Ebomycin like compounds belong to the macrocyclic lipid compounds of polyketones, which have strong stabilizing effects on polymeric microtubules. The mechanism of action is similar to that of paclitaxel, and they are superior to paclitaxel in terms of water solubility, cytotoxicity, and drug resistance. They are considered as newer products of paclitaxel and better anticancer drugs.

At present, the main preparation methods for ebomycin include chemical synthesis and biological synthesis. Due to the numerous steps and low yield, the chemical total synthesis method does not have a cost advantage compared to the biological fermentation method. Therefore, the biosynthesis method has become the mainstream direction for the production and preparation of ebomycin.

There are two main types of fermentation strains for preparing Ebomycin through biosynthesis methods: firstly, the original producing strain of Ebomycin, Fibrocystis, has a long growth period (8-16 hours) and extremely difficult genetic manipulation; The second type is yellow mucococcus, which heterologously expresses the bomycin synthesis gene. This type of strain has the advantages of short generation time (about 4 hours), vigorous growth, less pollution, and convenient genetic operation, which is conducive to subsequent engineering transformation. At present, there are few literature reports on the composition and optimization of the culture medium for the production of ebomycin by Staphylococcus aureus fermentation. Therefore, how to obtain a culture medium suitable for heterologous expression of ebomycin genes that can effectively improve fermentation yield and efficiency has become an urgent issue to be solved. Therefore, the CN105200090B patent has developed a culture medium for heterologous expression of the Ebomycin gene by fermentation of Staphylococcus aureus to prepare Ebomycin. The specific components and content of this culture medium are:

10 g/L casein peptone, 1.0 g/L MgSO₄·7H₂O, 2.07 g/L sodium 3- (N-morpholinyl) propionate, 5mL/L cis-9 octadecenoic acid methyl ester, 0.8g/L threonine, 0.5 g/L methionine, 1.0 g/L potassium propionate, 1 mL/L trace element solution, and 20 mL/L macroporous adsorption resin XAD-16.

The notable feature of this patent is the addition of cis 9-octadecenoic acid methyl ester, threonine, methionine, and potassium propionate. This composition and optimization can significantly improve the fermentation level and efficiency of ebomycin like compounds.

References

CN105200090B A Culture Medium for Heterologous Expression of Ebomycin Gene by Fermentation of Staphylococcus aureus to Prepare Ebomycin.