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Preparation of N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid (TAPS)
2022-02-15

TAPS is a widely used zwitterionic buffer in the fields of biochemistry and molecular biology with pH buffer range from 7.7 to 9.1, and is soluble in water (25g/50ml). It can be used as biobuffer in clinical diagnosis, such as biochemical diagnosis kits, DNA/RNA extraction kits, and PCR diagnostic kits. The molecular structure of TAPS is as follows: At present, there are few reports on the synthes...
Preparation of Tricine-SDS-PAGE Gel
2022-02-15

Tricine is a zwitterionic buffer for biochemistry and molecular biology, with a pH buffer range of 7.4-8.8. During SDS-PAGE to seperate small peptides, tricine is commonly used instead of glycine in the Laemmli buffer. Tricine-SDS-PAGE gel has superior performance for the identification of proteins with molecular weight below 10 kd. While conventional SDS-PAGE is used to separate these proteins, s...
Preparation methods of guanfacine hydrochloride
2022-02-15

Guanfacine hydrochloride is a selective alpha-2A adrenergic receptor agonist for the treatment of moderate to severe hypertension. It can also be used for the treatment of attention deficit hyperactivity disorder (ADHD). It is suitable for children and for adolescent that stimulant is not suitable, or invalid. Here we describe several methods for the synthesis of guanfacine hydrochloride and compa...
Guanidine Hydrochloride or Urea—Which is a Better Protein Denaturant?
2022-02-15

In general, guanidine hydrochloride and urea can be used as protein denaturant. Guanidine hydrochloride and urea are generally used to obtain an estimate of protein conformational stability (the difference between proteins in their native and denatured states). Is the result obtained using these two denaturants consistent? Let's take a look at the results of Monera et al. [1]. Although urea and gu...
GOOD’s Buffers——PIPES and HEPES
2022-02-15

Good’s buffer is especially dedicated to life science research that does not participate in and does not interfere with biochemical processes. Here we introduce two commonly used Good’s buffers PIPES and HEPES. PIPES PIPES has a pH buffer range from 6.1 to 7.5, is insoluble in water, but soluble in aqueous NaOH. PIPES differs from buffers containing bis(2-hydroxyethyl)amino groups (such as Bis-tri...
Bis(2-hydroxyethyl) amine Family Buffers
2018-03-20

The bis(2-hydroxyethyl)amine family buffers includes Bis-Tris, BES, and Bicine. Brief introduction of these three buffers are as follows. Bis-Tris Bis-tris is a zwitterionic buffering agent with pH range from 5.8 to 7.2. Bis-tris forms stable complex with Cu2+ and Pb2+ and a weak complex with many common metals, so formation constants should be taken into account when using this buffer in a soluti...
Preparation of BCIP/NBT Color Development Solution
2022-02-15

BCIP/NBT is one of the substrate combinations for alkaline phosphatase (AP) and is used to detect antigen bound to nitrocellulose or other membranes. Under the catalysis of AP, BCIP is hydrolyzed to produce a strong reactive product that reacts with NBT to form an insoluble dark blue to bluish-purple product. The corresponding kit can be used for enzymatic chromogenic reaction of the alkaline phos...
Fermentation Method for Producing L-2-aminobutyric Acid Using IPTG as Inducer
2022-02-15

L-2-aminobutyric acid is an unnatural amino acid that has the function of inhibiting the transmission of human nervous information, enhancing the activity of glucose phosphatase and promoting the metabolism of brain cells. At present, L-2-aminobutyric acid synthesis methods include chemical and biological methods. The chemical method involves the use of L-methionine as raw material to form L-2-ami...
Synergetic Effect of LiBF4 and LiODFB as Wide-temperature Electrolytes in Li-ion Battery
2022-02-15

Currently, Li-ion battery has been widely used in 3C device, electric vehicles, with environmentally friendly, no memory effect and so on. However, the narrow application temperature range limits its application in some areas and fields. At present, commercial Li-ion battery electrolytes have application temperature range of -20 °C ~ 60 °C and show a significantly lower capacity outside these temp...

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