Nucleic acids include deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), and are a class of biological macromolecules with genetic information. The study found that free DNA also contains very important genetic information. Therefore, in recent years, free DNA research has become a hot field in genomic molecular diagnostic research, and has been gradually applied to all stages from the initial diagnosis of tumor to treatment and development. However, the extraction technique of free DNA still needs further improvement.
The researchers developed a free DNA extraction and enrichment kit and a method for extracting free DNA, aiming to solve the technical problem that the organic solvent has certain toxicity in the existing free nucleic acid extraction method.
Free DNA extraction and enrichment kit
The kit includes sample lysate, binding solution, primary wash, secondary wash, eluent, and nanomagnetic beads:
Free DNA extraction method
The method do not use toxic reagents such as benzene and chloroform in the traditional method to minimize the damage to the experimental operators, fully comply with the modern environmental protection concept, and better meet the clinical use conditions; Lysis, denaturation and promotion of nucleic acid enrichment, most samples do not require additional use of proteinase K and sample lysate; It takes less time and is easy to operate for all binding, washing and elution processes at room temperature.
Reference
Free DNA extraction and enrichment kit and free DNA extraction method. CN109913447A, 2019-06-21.
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