MOPS,3-(N-Morpholino)propanesulfonic acid, is an important biological buffer, the buffer range is 6.5~7.9, which is widely used in biological and chemical experiments, such as electron transfer and phosphorylation in chloroplast thin layer, culture of Streptomyces coelicolus and the production of cephalosporins, and electrolyte system component in isoelectric focusing electrophoresis (IEF) of two-dimensional gel electrophoresis. So the preparation of MOPS Buffer is very important.

Existing technical routes typically require the use of an organic solvent to dissolve and provide a reaction atmosphere to ensure that the morpholine is on C3 of propanesulfonic acid. The use of a large amount of organic solvent not only increases the cost of production, but also the volatility of organic solvents, which adversely affects the health of the operator. How to recycle the used organic solvent is also a laborious and costly task. In addition, the use of organic solvents results in yields that are always around 70%, which in turn increases costs.

Wu Xiongfei, Zhang Fuxing, Xu Zhifeng, et al. Preparation of 3-morpholinepropanesulfonic acid. 2019-01-04, CN109134404A