Good's buffers are zwitterionic buffers, which are generally sulfamic acid-based organic compounds, their pH values are relatively stable and does not react with various enzymes and chemical reagents. It is mainly used in the field of biology and medicine. CAPS (N-Cyclohexyl-3-Aminopropanesulfonic Acid) is one of the commonly used Good's buffers in separation of basic drugs by enzymatic chemistry and HPLC. Therefore, its preparation is very important. In order to reduce the reaction conditions, it is generally carried out under the conditions of a catalyst and infrared light irradiation in the production process, while the production cost is increased. And at the same time, the purity of the preparation is difficult to be improved due to the instability of the reaction conditions. This article describes a low-cost method for preparing high purity CAPS.
(6) Washing with acetone for 3~7 times to remove cyclohexylamine (the ratio of acetone-washed acetone and crude 3-cyclohexylamine-1-propanesulfonic acid is 5:8), and finally washing 3-cyclohexylamine with distilled water for 3~7 times, and 500 mL of distilled water was used each time to obtain purified 3-cyclohexylamine-1-propanesulfonic acid.
The nanometer titanium dioxide catalyst with rare earth cerium is high in catalytic performance, and the reaction can be carried out without irradiation of infrared light; the sulfite and bisulfite are widely used, and are easily removed in the reaction; during the washing process, the PEEK can be effectively cleaned to ensure maximum acetone savings and lower production costs.
Yuan Xuesheng. Preparation method of 3-cyclohexylamine-1-propanesulfonic acid. 2016.11.16, CN106117088A
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