Tris(Hydroxymethyl) Aminomethane (TRIS) buffer solution is a biological reagent, which is a buffer solution commonly used in biological preparation and medical preparation. For example, it’s commonly used in peprotech recombinant cytokines and protein solubilization, and widely used as solvent for nucleic acid and proteins electrophoresis. Usually, it is used as a liquid, but it is not easy to store for a long time and the preparation is inconvenient. If it is made into a tablet, the above problem will be well solved.

Some researchers have provided a preparation method of TRIS buffer tablets which are ready to use, easy to store, simple and quick to prepare. The steps are as follows:
(1) TRIS, boric acid, EDTA, cross-linked amylose (weight ratio: 54:22~23:3.0~3.5:3.3~4), pulverized to make into particles with 100~200 mesh; The main material trishydroxymethylaminomethane is in the grade of pharmacopoeia; Excipients boric acid and EDTA are AR grade, crosslinked amylose and microcrystalline cellulose are biological grade;
(2) mixing and stirring evenly;
(3) Tablet press, pressure 2~4 tons;
(4) Weighing and testing;

(5) Packaging.

The pH of the TRIS buffer solution varies with temperature greatly, making it more difficult to formulate the solution. The buffer tablet provided by the method is simple and easy to use, and can be quickly opened and disintegrated as long as the tablet is put into the quantitative water, thereby obtaining a buffer solution with an accurate pH value, saving the user's manpower and material resources and improving work efficiency. At the same time, it is easy to store, carry and transport. The storage period is up to 3~5 years, and suitable for industrial production.

Edited by Suzhou Yacoo Science Co., Ltd.

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