4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) is a hydrogen ion buffer which has a good buffering capacity in the pH range of 6.8~8.2 and can control a constant pH for a long time. It can be used in a wide range of biochemical reactions, such as, in the research of organelles and highly denatured pH-sensitive proteins and enzymes, as well as biochemical diagnostic kit, DNA/RNA extraction kits and PCR diagnostic kit. Below we will list the common used recipes.
Reagent |
Amount to add (for 1 L) |
Final concentration |
NaCl |
6.72 g |
115 mM |
CaCl2 |
133 mg |
1.2 mM |
MgCl2 |
114 mg |
1.2 mM |
K2HPO4 |
418 mg |
2.4 mM |
HEPES |
4.77 g |
20 mM |
H2O |
to 1 L |
Reagent |
Final concentration |
NaCl |
116 mM |
KCl |
4 mM |
MgCl2 |
1 mM |
CaCl2 |
1.8 mM |
Glucose |
25 mM |
HEPES acid |
10 mM |
Reagent |
Final concentration |
NaCl |
150 mM |
Tween-20 |
0.005% (v/v) |
EDTA |
3 mM |
HEPES pH (7.4) |
10 mM |
Reagent |
Final concentration |
NaCl |
10 mM |
Na-β-glycerophosphate |
20 mM |
EDTA |
1 mM |
HEPES pH (7.5) |
50 mM |
Na3VO4 |
0.1 mM |
NaF |
50 mM |
PMSF |
1 mM |
DTT |
1 mM |
NP-40 |
0.1% |
Protease inhibitor cocktail |
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