Phenylmethylsulfonyl fluoride, referred to as PMSF, CAS number: 329-98-6, is one of the superior products of Yacoo Science, the model number is B0009. Phenylmethylsulfonyl fluoride (PMSF) is widely used as a protease inhibitor in biochemical and molecular biology experiments. This article will introduce the nature and application of this important biochemical reagent.

Phenylmethylsulfonyl fluoride (PMSF) is white needle-like crystals, not easily soluble in water, but very unstable in aqueous solution, easily decomposed and sensitive to humidity, and easily soluble in methanol, ethanol and isopropanol.

It should be noted that phenylmethylsulfonyl fluoride is toxic and corrosive. It is very destructive to respiratory mucosa, eyes and skin. It can be fatal by inhalation, swallowing or skin absorption. Therefore, personal protection must be done during use, protective gloves and safety glasses must be worn, and always operated in a chemical fume hood. At the same time, it may be harmful to the environment and water bodies, so please pay attention to the hazardous waste treatment after use.

The uses of phenylmethylsulfonyl fluoride (PMSF):

Phenylmethylsulfonyl fluoride is a non-organophosphorus organic compound and a broad-spectrum esterase/protease inhibitor that can inhibit serine proteases such as trypsin and chymotrypsin; also It can inhibit the activity of cysteine protease and acetylcholinesterase. Phenylmethylsulfonyl fluoride can also be condensed with carbonyl compounds to prepare olefins.

According to published literature, phenylmethylsulfonyl fluoride can inhibit or enhance the occurrence of delayed neurotoxicity caused by organophosphorus compounds, and it also protects liver necrosis and cerebral ischemia. Researchers at Shandong University have proved through experiments that phenylmethylsulfonyl fluoride can effectively prevent and treat acute liver injury induced by acetaminophen (APAP) and can be used to prepare drugs for the prevention and treatment of drug-induced liver injury.