NSP-DMAE-NHS, CAS No.: 194357-64-7, is one of the most widely used chemiluminescent markers,which plays an important role in chemiluminescent immunoassay.

Chemiluminescence immunoassay (CLIA) is a combination of highly sensitive chemiluminescence assay technology and highly specific immune response. It is used for the detection and analysis of various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins and drugs.

There are many kinds of chemiluminescence systems used in clinical tests. The most important ones are luminol and its derivatives, NSP-DMAE-NHS, helical adamantane-1,2-dioxyethane and its derivatives, and electrochemiluminescence systems. Among them,NSP-DMAE-NHS are considered to be ideal luminescent substrates.

Compared with other chemiluminescence systems, what are the unique advantages of NSP-DMAE-NHS ?

NSP-DMAE-NHS luminescence system is simple, does not require a catalyst.

Under alkaline conditions, acridinium ester molecules can be attacked by hydrogen peroxide to generate dioxins. Ethane, dioxyethane is unstable and decomposes into CO2 and an electron-excited N-methylacridone, which emits light having a wavelength of 430 nm when it returns to the ground state.NSP-DMAE-NHS luminescence system is simple and does not require an enhancer, reduces background luminescence, improves sensitivity and has less interference.

NSP-DMAE-NHS luminescence system have high luminous efficiency and high intensity.

 The NSP-DMAE-NHS chemiluminescence is flash luminescence, which has unique advantages other techniques in the field of chemiluminescence immunoassay. The NSP-DMAE-NHS chemiluminescence has the highest emission intensity after adding the initiator for 0.4s, and the half-life is 0.9s, which usually five times or more than luminol .

NSP-DMAE-NHS luminescence system has less interference factors,low background and the signal-to-noise ratio is high.

From the mechanism of light emission, NSP-DMAE-NHS luminescence system have the following characteristics:in the luminescent reaction, before the formation of the electronically excited intermediate, the non-luminescent substituent moiety attached to the acridine ring is detached from the acridine ring, ie, does not emit light. The portion is separated from the light-emitting portion, and thus its luminous efficiency is substantially unaffected by the substituent structure.

On the other hand, the molecular weight of NSP-DMAE-NHS is small, which is easy to be firmly linked with protein through chemical bond。It has little influence on the conformation of the antibody after conjugation (thus ensuring its good reactivity in subsequent immunoassay), and the stability of the conjugates is good.

Related links:luminol 、NSP-DMAE-NHS

References: CN105158235B,CN105628914B,CN108997475A