HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, its CAS number: 7365-45-9, HEPES is a zwitterionic buffer with a pka7.45 at 25 ℃ and a pH buffering range of 6.8-8.2.

HEPES-Na,4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, sodium salt, its CAS number is: 75277-39-3. HEPES-Na and HEPES are very important biological buffers and are widely used in biopharmaceuticals, medical diagnostics and other fields.

The relationship between HEPES-Na and HEPES:

HEPES-Na is also known as organic HEPES base, it and HEPES is a conjugate acid-base relationship. They are essentially the same, but the pH of them substances after dissolution is different.

The method of Preparing HEPES-Na:

According to published literature, HEPES-Na can be synthesized by 4-hydroxyethylpiperazine and sodium vinylsulfonate, or by high-pressure synthesis of isethionic acid, sodium hydroxide and 4-hydroxyethylpiperazine. The most extensive preparation method required by biological buffers is the reaction of HEPES with NaOH to produce HEPES-Na. The specific preparation steps are as follows:

Firstly, under the protection of nitrogen, HEPES and NaOH solids or solutions with a purity of 90-99% are reacted for 0.2-1 hour, the reaction temperature is 20-100°C. After the reaction is completed, the resulting product is decolorized, filtered, concentrated, Operations such as water removal, crystallization, washing, and drying are used to obtain HEPES-Na.

The preparation method is simple in process and convenient in operation, and the obtained HEPES-Na product has high yield and high product purity, and can meet the purity requirements of the biological buffer.

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